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Additionally, more than one receptor type and different G-protein coupling of neuropeptide receptors in different tissues lead to variable effects of the same neuropeptides in different tissues/cell types [24,25,26,27,28,29,30,31,32,33,34,35,36]

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These organic broccoli seeds have 3x more glucoraphanin (sulforaphane precursor) than typical broccoli seeds (some broccoli sold on the market actually have none!)

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After a 48-hour incubation with the drugs, the medium was replaced with Seahorse XF base medium (Agilent Technologies, USA) and incubated for 1 h at 37 C in a CO 2 -free environment